Fig. 2.

Representative 2DE protein patterns from control and microgravity exposed mouse hypothalamus. Control mice were maintained under normal condition in cages while other group of mice was kept for microgravity exposure by suspending them by their tail for 7 days as described in materials and methods. Hypothalamus was dissected and homogenized in 2DE extraction buffer. Approximately 150 μg of protein was used for 2DE and stained with Sypro Ruby. Images were captured and differentially expressed proteins were identified by visual analysis. Differentially expressed proteins spots are marked on the images with arrows