Fig. 3. Effect of IL-8 on induction of ICAM-1, VCAM-1, and Cox-2.

U-937 cells were stimulated with different concentrations of IL-8 and 100 pM of TNF for 24 h at 37 °C, CO₂ incubator. 100 μg of extract proteins were analyzed in 10% SDS-PAGE, and ICAM-1 (A), VCAM-1 (B), or Cox-2 (C) were detected by Western blot. All these gels were reprobed with anti-tubulin monoclonal antibody. U-937 cells were co-transfected with the Cox-2-luciferase plasmid and β-galactosidase gene. After 3 h of transfection, cells were stimulated with different concentrations of IL-8 or TNF for 24 h. The luciferase (D) and β-galactosidase enzymes activity was measured from cell extracts.