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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: J Biol Chem. 2004 Dec 9;280(8):7010–7021. doi: 10.1074/jbc.M410994200

Fig. 5. Effect of IL-8 on MEK and JNK activation.

Fig. 5

U-937 cells were stimulated with different concentrations of IL-8 and 100 pM TNF for 2 h (panel A1) or at different times with 100 ng/ml IL-8 as indicated (panel A2) at 37 °C. The cells were used to detect phosphorylated MAPK (MEK). Both blots were reprobed with anti-ERK1 antibody. For JNK, U-937 cells were stimulated with different concentrations of IL-8 and 100 pM TNF (panel B1) or for different times with 100 ng/ml IL-8 (panel B2) at 37 °C for 2 h. Then JNK was assayed from cell extracts as described under “Experimental Procedures.” 50 μg of extract proteins from the same treatments were detected JNK1 by Western blot.