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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Circ Res. 2008 Jul 24;103(5):467–476. doi: 10.1161/CIRCRESAHA.108.177105

Figure 1. Visceral AT from obese mice contains more inflammatory cells than that from lean controls.

Figure 1

SVCs from peri-epididymal AT from lean and obese C57BL/6J animals were labeled with conjugated antibodies to F4/80, CD3 (I), CD4, CD8 (II), CD11c, and B220 (III), and quantified by flow cytometry. The graphs represent differences between mice on LF (lean) and HF (obese) diets, calculated by Student’s t-test. *p<0.05 vs LF.

Immunostaining of peri-epididymal fat tissue with rat anti-mouse CD45 (A–C), Mac-3 (E–G), CD3 (I–L), and I-Ab (N-P) antibodies was performed as described. Graphs (D, H, M, Q) represent the numbers of positive cells in each group, and the differences were calculated by Student’s t-test. Original magnification: x100 for A–B, E–F, I–J, N–O; x400 for C, G, L, and P. #p<0.05 vs LF; n=5–8.