Fig. 5.
cFLIPL is necessary for GLUT1-mediated prevention of apoptosis and induction of proliferation. Knockdown (kd) of cFLIPL expression in GLUT1 cells (A) resulted in decreased PCNA expression (B) and increased caspase 3 activity (C) and had little effect in control cells. Knockdown of cFLIPL expression resulted in a decrease in the number of live (D) and an increase in the number of dead (E) GLUT1 cells, again with little effect on control cells. cFLIP mRNA levels were normalized to those of GAPDH, and PCNA protein levels were normalized to those of β-tubulin. shRNA, short hairpin RNA.