FIGURE 2.

Effect of induced adhesion of K562 cells on their erythroid differentiation stimulated by hemin. Erythroid differentiation was stimulated by hemin (50 μm) in K562 cells in the presence or absence of TNIIIA2 (75 μg/ml). After culturing for 3 days (A) or the indicated periods (B), cells were stained with o-dianisidine, as described under “Experimental Procedures.” One of three individual experiments is shown in A. The percentage of the number of hemoglobinized cells, relative to the total number of cells, is shown in B. Data represent the mean ± S.E. of triplicate determinations. *, p < 0.05 compared with control.