FIGURE 3.

l-[¹⁴C]Citrulline production in the presence of NADPH-regenerating system. Measurement of the conversion of l-[¹⁴C]arginine to l-[¹⁴C]citrulline was performed using an NADPH-regenerating system containing glucose 6-phosphate (125 mm), NADP (12.5 mm), and 1 unit of glucose-6-phosphate dehydrogenase. 1st bar, positive control, nNOSr (30 nm); 2nd bar, MT (150 μg); 3rd bar, MT (150 μg) + nNOSr (30 nm). Values from control incubations containing all ingredients except enzyme were subtracted from all data points. Data were expressed as the mean ± range of values, of experimental data points from two independent experiments, each run in duplicate.