FIGURE 7.

ZBTB5 stimulates cell proliferation and increases the number of cells in S-phase. A, B, FACS analysis of cell cycle progression. HEK293A cells were transfected with ZBTB5 expression vector or control vector, cultured, and stained with propidium iodide. Cell proliferation was measured by FACS. Alternatively, the cells were transfected with siRNA against ZBTB5 mRNA, and cell cycle progression was analyzed. N.C., scrambled siRNA negative control. C, D, MTT assay of HEK293A cells grown for 1, 2, 3, and 4 days. The cells were transfected with either control pcDNA3 vector or pcDNA3-FLAG-ZBTB5 expression vector and analyzed for cell growth. Alternatively, the cells were treated with either negative control siRNA or siZBTB5 RNA. All assays were performed in triplicate. Error bars are included but are too tight to see.