FIGURE 3.

Activation of sGC by GTN in the presence of WT-ALDH2 (A) and E268Q-ALDH2 (B). Purified sGC (50 ng) was incubated at 37 °C for 10 min with 0.1 mm GTN and the indicated concentrations of WT- or E268Q-ALDH2 in 0.1 ml of 50 mm TEA/HCl (pH 7.4), containing 0.5 mm [α-³²P]GTP (∼250,000 cpm), 3 mm MgCl₂, 0.1 mm DTPA, and 1 mm cGMP in the absence and presence of DTT (2 mm) and SOD (1,000 units/ml). The samples were analyzed for [³²P]cGMP as described under “Experimental Procedures.” The data are the mean values ± S.E. of three independent experiments.