TABLE 2.

Formation of GTN-derived NO relative to denitration catalyzed by WT- and E268Q-ALDH2

Initial rates of NO formation were divided by the initial rates of 1,2-GDN formation catalyzed by 10–100 μg/0.1 ml of WT- and E268Q-ALDH2. The data are the mean values ± S.E. of relative NO formation (expressed as percentages of 1,2-GDN formation) calculated from three independent experiments.

ALDH2	vi(NO)/vi(1,2-GDN) × 100
	WT		E268Q
	−NAD	+NAD	−NAD	+NAD
10 μg/0.1 ml	10.6 ± 0.55	13.4 ± 1.61	8.69 ± 2.43	31.7 ± 5.83a
25 μg/0.1 ml	6.39 ± 0.52	8.11 ± 0.84	8.12 ± 1.29	48.2 ± 6.59a
50 μg/0.1 ml	6.97 ± 0.34	6.34 ± 0.43	8.03 ± 0.48	37.4 ± 4.16a
100 μg/0.1 ml	6.06 ± 0.68	6.53 ± 1.29	9.12 ± 0.49	40.3 ± 1.85a

^ap < 0.001 vs. WT ± NAD and E268Q without NAD (analysis of variance, Holm-Sidak method).