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Pr3 activity at the surface and in the supernatant of activated neutrophils. About 25% of the total Pr3 activity is released spontaneously when cells are suspended in PBS for at least 120 min, and no further release occurs following incubation with 1.5 m NaCl, whereas almost all the membrane-bound HNE is released into the supernatant after incubation with NaCl.
