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. 2009 Apr 27;284(30):19903–19914. doi: 10.1074/jbc.M808141200

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Requirement of multimerization and nuclear export properties for the inhibition of particle production by INI1-S6-(183–294). A, location of DD5 and DD6 mutations with respect to NES. B, confocal microscopy of GFP-fused S6 and DD5 and DD6 mutants. C, subcellular localization of S6 and DD5 and DD6 mutants. Values are expressed as percentages. D, intracellular, virion-associated, and ratio of virion-associated to intracellular p24 in the presence of wild type INI1 or S6, DD5, and DD6 mutants. E, expression of INI1, S6, DD5 and DD6 proteins in 293T cells. F, coimmunoprecipitation (IP) of YFP-IN with HA-tagged S6, DD5, and DD6. Inputs of YFP-IN and HA-tagged proteins are shown. WB, Western blot; PI, propidium iodide.