FIGURE 5.

Mutant GCN5 fails to acetylate PGC-1β and shows little impairment of transcriptional activity. A, HEK 293 cells were transfected with the indicated plasmids encoding either wild-type GCN5 or mutant GCN5. FLAGHA-PGC-1β was overexpressed and immunoprecipitated using FLAG antibody linked to agarose beads. Detection of protein and acetylation levels was completed with Western blot as previous. B, HEK 293 cells were transfected with 5XUAS as well as GAL4-Errα and other indicated plasmids. Luciferase activities were measured as described under “Experimental Procedures.” Values are representative of mean ± S.E. of two experiments each in triplicate Statistical significance was determined by two-tailed unpaired students t test. *, p < 0.05 PGC-1β versus PGC-1β plus GCN5.