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. 2009 May 28;284(30):19953–19960. doi: 10.1074/jbc.M109.011171

FIGURE 2.

FIGURE 2.

Transport selectivity and kinetics of XT2 co-expressed with ACE2. A, substrate selectivity of XT2 co-expressed with ACE2 in X. laevis oocytes. Na+-Cl−-dependent uptake selectivity of l-amino acids (0.1 mm) was assayed for 10 min. Amino acids are abbreviated with single-letter codes. B and C, concentration dependence of the XT2-ACE2-mediated transport in oocytes. Curves corresponding to Michaelis-Menten kinetics were fitted to Na+-Cl−-dependent uptake of l-Ile (B) and Gly (C). Data represent means of 18–39 oocytes ± S.E. from 3–5 independent experiments. D, transport induced by XT2 co-expressed with ACE2 is Na+- and Cl−-dependent. Data are means of 13–16 oocytes ± S.E. from two independent experiments. E, transport of l-Ile was not influenced by pH. The transport of l-Ile was assayed in the presence of Na+ and Cl− at different pH values. Shown are means from eight oocytes ± S.E. The open bars show transport rate of XT2-expressing, black bars of XT2 + ACE2-expressing, and gray bars of ACE2-expressing oocytes (D and E). ns, not significant.