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Effect of acute sublethal osmotic stress (550 mosmol/kg H2O) on ZAC1 protein expression in IMCD3 cells. Cell lysates (0–72 h) were analyzed by Western blotting. Data depict the mean ± S.E. from three Western blots (50 μg of total protein per lane, n = 3). A representative Western blot including β-actin loading control is shown.
