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. 2009 May 7;284(30):19974–19981. doi: 10.1074/jbc.M109.001792

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Overexpression of ZAC1 induces SDH down-regulation. A, expression of mouse ZAC1 in IMCD3 cells maintained at isotonic conditions results in the down-regulation of SDH levels. A representative Western blot including β-actin loading control is shown. B, expression of human ZAC1 in mouse ZAC1-silenced cells leads to SDH down-regulation under hypertonic stress conditions. Upper, agarose/qPCR analysis showing hZAC1 expression in silenced clone H. Lower, representative Western blot including β-actin loading control showing down-regulation of SDH expression in clone H under hypertonic conditions compared with no human ZAC1 expression.