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Densities of the FAD moieties in WT, C166A, and H104A BBE. Each panel shows two perpendicular views of the electron density of the cofactor including the amino acids 104 and 166 observed in the three BBE variants. A, Fo − Fc density map of WT BBE contoured at 5.0 σ. B, 2Fo − Fc density map of C166A BBE contoured at 3.0 σ. C, 2Fo − Fc density map of H104A BBE contoured at 1.5 σ.
