FIGURE 10.

Structural model of MCT1 associated with embigin illustrating the site of DIDS cross-linking. Details of the modeling strategy are given in the text. The proteins are represented as ribbons (red, N-MCT1; blue, C-MCT1; green, embigin). The lysine residues involved in cross-linking (Lys³⁸ of MCT1, and Lys¹⁶⁴ and Lys¹⁶⁰ of embigin) are indicated as magenta sticks, whereas the cysteine disulfide bridges of the two immunoglobulin domains of embigin are shown as yellow sticks. DIDS is shown as a space-filling representation, with standard atom coloring.