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EHD4 interacts with CDH23 in yeast. A, when a prey plasmid encoding EHD4 cDNA was transformed into the control-bait yeast, there was no growth on SD-LTHA plates because EHD4 and the control-bait proteins failed to interact. B, when the EHD4-prey plasmid was transfected into the CDH23-bait yeast, there was a bait/prey interaction, and histidine was produced, as evidenced by the growth on SD-LTHA-selective plates.
