FIGURE 2.

Expression of EHD4 mRNA in the cochlea. In situ hybridization was used to detect the expression of EHD4 mRNA in the cochlea. WT and EHD4-KO mouse cochlear tissues were stained with the sense (A for WT and C for EHD4-KO) or antisense EHD4 RNA probes (B for WT and D for EHD4-KO). Only the antisense EHD4 RNA probe stained OHCs, as well as IHCs in WT, but not in EHD4-KO mouse cochlear tissue, thereby demonstrating that EHD4 is present in both populations of sensory receptor cells.