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. 2009 Jun 1;284(30):20121–20129. doi: 10.1074/jbc.M109.025668

FIGURE 6.

FIGURE 6.

A, EHD1 possibly compensates for the loss of EHD4 in EHD4-KO cochleae. Cochlear lysates from EHD4-KO and WT mice were mixed with SDS-Laemmli sample buffer and run on 7.5% SDS-PAGE, then probed with anti-EHD4, anti-EHD1, and anti-EHD2 antibodies, respectively. EHD1 expression in EHD4-KO cochlea is higher than EHD1 expression in WT cochlea but the expression of EHD2 is similar in WT and EHD4-KO cochlea. Hsc70 was used as a loading control. The lane between EHD4-KO cochleae and WT lung is empty. B, semi-quantitative analysis of EHD proteins. Densitometric analysis of (upper image) EHD4 and EHD1 proteins and (lower image) EHD4 and EHD2 proteins in WT (n = 4) and EHD4-KO cochleae (n = 4) blotted with (upper image) anti-EHD1 antibody and (lower image) anti-EHD2 antibody, respectively. EHD1 expression in EHD4-KO cochleae is more than double the EHD1 expression in a WT cochlea. In contrast, EHD2 expression is similar in both WT and EHD4-KO cochleae.