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EHD3 protein levels do not change in EHD4-KO cochleae. Cochlear lysates from EHD4-KO and WT mice were mixed with SDS-Laemmli sample buffer and run on 7.5% SDS-PAGE, then probed with anti-EHD4, anti-EHD3, and anti-Hsc70 antibodies sequentially. EHD3 protein level is similar in EHD4-KO and WT cochleae. Hsc70 was used as a loading control. Lysates from EHD4 WT lung were used as an additional control.
