. 2009 Jun 2;284(30):20147–20154. doi: 10.1074/jbc.M109.005769

TABLE 2.

Rb⁺ uptake activity, SCH28080 sensitivity, and apparent Rb⁺ affinity of N-terminally deleted H,K-ATPase β-mutants (Fig. 3)

	Normalized Rb⁺ uptake activity (5 mm RbCl)^a^,^b	Residual Rb⁺ uptake activity in presence of SCH28080 ^a^,^c		Apparent Rb⁺ affinity (K_0.5)^d
	Normalized Rb⁺ uptake activity (5 mm RbCl)^a^,^b	10 μm SCH28080	100 μm SCH28080	Apparent Rb⁺ affinity (K_0.5)^d
	%	%		mm
HKαS806C/βWT	100 ± 3.9	21 ± 2.0	6 ± 0.7	0.66 ± 0.09
HKαS806C/βΔ4	96 ± 3.3	21 ± 1.7	14 ± 1.8	0.62 ± 0.14
HKαS806C/βΔ8	95 ± 4.4	18 ± 2.0	13 ± 2.3	0.70 ± 0.18
HKαS806C/βΔ13	77 ± 4.0	28 ± 2.3	25 ± 2.6	0.65 ± 0.12
HKαS806C/βΔ29	83 ± 3.8	27 ± 2.2	20 ± 1.7	0.52 ± 0.10
Uninjected	4 ± 0.4

^a The values are the means ± S.E. of 40–50 oocytes from three or four different oocyte batches.

^b The data were normalized to Rb⁺ uptake of HKαS806C/βWT, corresponding to 32 pmol/min/oocyte.

^c The data were normalized to Rb⁺ uptake at 5 mm RbCl in the absence of SCH28080 for each construct after subtraction of the mean Rb⁺ uptake of uninjected oocytes.

^d The values are the means ± S.E. of 10–20 oocytes.