FIGURE 1.

Substrate activity of truncated forms of eEF1A. Fragments of eEF1A were produced as described under “Experimental Procedures” and used in ¹⁴C-glucosylation assay with Lgt1. Arrowheads on the left of each SDS-PAGE lane indicate approximate molecular mass of the peptide (in kilodaltons). Predicted structural views of each truncated molecule are shown at the bottom. Black arrows indicate the position of serine 53 residue, known to be targeted by glucosylation. The protein with a molecular mass of 29 kDa represents G domain of eEF1A. Structural scheme of the 19-kDa fragment is turned 180° to simplify the presentation of the molecule.