FIGURE 4.
Time course of glucosylation of eEF1A-derived substrates by Lgt1. 1 μm of each eEF1A-derived decapeptide (open squares), eEF1A-derived 25-mer peptide (open diamonds), G domain of eEF1A (open circles), and purified yeast eEF1A (filled squares) was incubated with 70 nm Lgt1 and 10 μm UDP-[14C]glucose at 37 °C for 3, 10, and 30 min. Thereafter, autoradiography was performed, and the data were quantified as described under “Experimental Procedures.” Data are given as the mean of at least three independent measurements.