FIGURE 8.
TNF-α-induced enhancement of PARP-1 binding to the G−172 → T region and transcription activity of MOR gene in SH-SY5Y cells. Nuclear protein or cell lysate in SH-SY5Y was prepared at respective times after stimulation with TNF-α (10 ng/ml). A, PARP-1 binding to the G−172 or the T−172 probe after the stimulation of TNF-α was investigated by EMSA. B, SH-SY5Y cells transfected with pGL3G−172 → T or with pGL3G−172 → T TK were stimulated by TNF-α and were prepared 12 h after TNF-α stimulation for luciferase assay. Luciferase activity in T−172 was represented as relative value against that in G−172. The error bars indicate S.D. derived from three independent experiments. β-galactosidase activities, as controls (CON) for transfection efficiency, were used to normalize the data. The asterisks indicate significantly difference (**, p < 0.01).