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. Author manuscript; available in PMC: 2010 Jul 14.
Published in final edited form as: Biochemistry. 2009 Jul 14;48(27):6390–6401. doi: 10.1021/bi900621w

Figure 4. Atg14 is not necessary to mediate the interaction of Vps15 and Gpa1.

Figure 4

Detergent-solubilized extracts from wildtype (WT) and atg14Δ mutant cells expressing the indicated Flag fusion proteins and Gpa1 fused to a Myc epitope were incubated with Flag resin, eluted with 3X Flag peptide, resolved by 10% SDS-PAGE, and analyzed by immunoblotting with antibodies against Flag, Myc, and G6PDH (Load control). IP, immunoprecipitation. WD, WD domain. KD, kinase domain. ID, intermediate domain. *, indicates protein of interest.