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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: J Neurosci Methods. 2008 Sep 19;176(2):172–181. doi: 10.1016/j.jneumeth.2008.09.013

Figure 1.

Figure 1

NovaRed stained PV-ir interneurons in mPFC before and after LMD. A, photograph of Cresyl violet-stained frontal cortex with dorsal to the right and ventral to the left of the image. B, higher magnification (squared area in A) of Nissl staining showing the laminar architecture of mPFC. Dashed lines delineate cortical layers. C and D, photographs of NovaRed-staining at low magnifications showing the area of interest in the mPFC. E and F, photographs from D (squared area) showing the PV-ir interneurons (arrows point to the cells of interest) before (E) and after (F) laser cut. The PV-ir interneurons were stained in brown/red color. Scale bar in A = 1600 μm for A, 400 μm for B and C, 200 μm for D, and 50 μm for E and F.