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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Biochem J. 2008 Jan 15;409(2):525–533. doi: 10.1042/BJ20071263

Fig. 4. Zizimin1 N-terminal half inhibits binding of the C-terminal half to Cdc42.

Fig. 4

Flag-tagged zizimin1 N- or C-terminal halves were purified from Cos7 cells by Flag IP and peptide elution (coomassie-stained gel is displayed on the right). Binding of the C-terminal half (at 20 nM) to Cdc42 (at 200 nM) was assayed in the absence or presence of 100 nM N-terminal half. Bound C-terminal half was detected by Western blotting with anti-Flag and quantified by densitometry (left panel). Values are mean ± SD (n=3) relative to control (without N-half).