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. 2009 May 11;284(29):19228–19236. doi: 10.1074/jbc.M109.008318

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Endocytosis of Jen1-HA in rsp5/npi1 mutant cells. Parental SP1 (WT) and SP2 (rsp5/npi1) cells harboring chromosomally encoded Jen1-HA were induced for 4 h in lactic acid before glucose addition. A, lactic acid uptake was measured at the times indicated after the addition of glucose (2%). The results are percentages of initial activities. □, wild-type; ▲, rsp5/npi cells. B, protein extracts were prepared at the same time points and analyzed by Western immunoblotting with an anti-HA antibody. The blots were reprobed with an anti-phosphoglycerol kinase (PGK) antibody to provide loading controls.