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. 2009 May 11;284(29):19228–19236. doi: 10.1074/jbc.M109.008318

FIGURE 4.

FIGURE 4.

Effect of Lys to Arg mutations on the stability of Jen1-GFP. W303-1A jen1Δ cells transformed with either pSP2 (JEN1-GFP), pSP3 (JEN1-K9R-GFP), or pSP4 (JEN1-K338R-GFP) were induced for the production of Jen1-GFP in lactic acid for 4 h at 30 °C. A, acid lactic uptake was measured at the times indicated after the addition of 2% glucose. The results are percentages of initial activities. ■, Jen1-GFP; ▿, Jen1-K9R-GFP; ▵, Jen1-K338R-GFP; B, protein extracts collected at the same time points were analyzed by Western immunoblotting with an anti-GFP antibody. The blots were reprobed with an anti-phosphoglycerol kinase (PGK) antibody to provide loading controls. C, microscopy images of Jen1-GFP in living cells at the time points indicated after the addition of 2% glucose. WT, wild type (Jen1-GFP).