TABLE 1.

Steady-state NO synthesis and NADPH oxidation rates of wild-type and mutant nNOS

Rates were measured at 25 and 10 °C, as described under “Experimental Procedures.” The NO synthesis rate was measured using l-Arg as substrate. Values (min⁻¹) represent the mean ± S.D. of three independent measurements with two preparations of each enzyme. WT, wild type.

Enzyme	NO synthesis	NADPH oxidation rates
		+CaM +Arg	−CaM +Arg	+CaM −Arg
	min−1		min−1
Activities at 25 °C
nNOS WT	56 ± 2.5	115 ± 7	8 ± 0.2	346 ± 6
E762N	105 ± 8.7	308 ± 16	94 ± 7	142 ± 3
Activities at 10 °C
nNOS WT	21 ± 1.5	40 ± 4	5 ± 0.5	124 ± 10
E762N	34 ± 3	107 ± 11	27 ± 3	47 ± 4