TABLE 2.
Equilibrium midpoint potentials (Em) versus standard hydrogen electrode of nNOSr proteins
Midpoint potentials of each flavin (ox/sq and sq/hq) were determined for the nNOSr proteins by potentiometric titration at 15 ± 1 °C, as described under “Experimental Procedures.” WT, wild type.
| Sample | Condition | FMN |
FAD |
||
|---|---|---|---|---|---|
| ox/sq | sq/hq | ox/sq | sq/hq | ||
| WT nNOSr | −CaM | −49 ± 5 | −274 ± 5 | −232 ± 7 | −280 ± 6 |
| +CaM | −30 ± 4 | −267 ± 5 | −234 ± 6 | −284 ± 9 | |
| E762N nNOSr | −CaM | −156 ± 9 | −187 ± 8 | −253 ± 4 | −275 ± 9 |
| +CaM | −153 ± 8 | −174 ± 10 | −250 ± 7 | −274 ± 8 | |