FIGURE 5.

Inhibition of MA-MT activities in vivo. Bacteria in culture were treated with various concentrations of SADAE, and the neosynthesis of mycolic acids in vivo was followed by [1-¹⁴C]acetate labeling. A, M. smegmatis/pMV261∷hma. Only points after 6 h of incubation are displayed for reasons of clarity; similar profiles were obtained after 3 h of incubation. B and C, M. tuberculosis. C, α-MA methyl esters scraped off the plate shown in B and separated by TLC on an AgNO₃-impregnated silica gel plate. D and E, relative labeling intensities for the various molecular species assessed on plates B and C, respectively. All runs were performed with dichloromethane (A and C) or petroleum ether/diethyl ether (9:1) (five times) (B), and radiolabeled spots were detected by phosphorimaging. F, fatty acid methyl esters; K, keto-; E, epoxy-; H, hydroxy-; M, methoxy-MA methyl esters. α₁′, dicyclopropanated; α₂′, monoethylenic-monocyclopropanated; α₃′, diethylenic α-MA methyl esters.