FIGURE 3.

Effects of hypoxia and reoxygenation on TNFR2 mRNA and protein levels. HDMECs were subjected to hypoxia followed by reoxygenation and TNFR2 mRNA (A) and protein (B and C) were quantified as in Figs. 1 and 2. Note that mRNA levels recover sooner than those of protein, which actually fall further upon reoxygenation, and that mRNA but not protein levels transiently overshoot the levels in normoxic cells. Data represent one of three experiments with similar results, although the kinetics varied among different experiments. TNFR2 mRNA was significantly reduced by 6 h (p < 0.05). Levels remained significantly lower (p < 0.05) until 6 h of re-oxygenation whereby they significantly increased by 9 h and returned to baseline by 18 h.