FIGURE 7.

Effects of FOXO3a overexpression on the response of TNFR2 promoter-reporter genes. A, partial sequence of the 5′-flanking region of the human TNFR2 gene, identifying regions containing the FOXO3a binding element (box (a)) and a putative HIF-1 binding element (box (b)) used to construct promoter-luciferase reporter genes as described under “Experimental Procedures.” B, FOXO3a overexpression induces an increase in luciferase activity, corrected for transfection efficiency, in a promoter-reporter construct incorporating the FOXO3a binding element but not the putative HIF-1 binding element in transiently transfected HDMECs. Data represent one of three experiments with similar results.