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. 2009 May 27;284(29):19380–19391. doi: 10.1074/jbc.M109.004531

TABLE 2.

The dissociation constant (K_D) obtained from the Elastin and HTE binding by Lig proteins determined by ELISA

Truncated Lig	K_D
Truncated Lig	Elastin	HTE	1–18HTE	17–27HTE	27–36HTE
LigBCon	166 ± 38 nm	ND^a	ND^a	ND^a	ND^a
LigBCon1–3	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCon4–7	181 ± 33 nm	ND^a	ND^a	ND^a	ND^a
LigBCon4	179 ± 29 nm	475 ± 80 nm	NB^b	501 ± 51 nm	NB^b
LigBCon5	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCon6–7′	NB^b	ND^a	ND^a	ND^a	ND^a
LigAVar	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCen	101 ± 11 nm	ND^a	ND^a	ND^a	ND^a
LigBCen1	189 ± 21 nm	ND^a	ND^a	ND^a	ND^a
LigBCen7′–8	750 ± 56 nm	824 ± 17 nm	NB^b	833 ± 13 nm	NB^b
LigBCen9	1230 ± 15 nm	1390 ± 11 nm	NB^b	1540 ± 351 nm	NB^b
LigBCen10	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCen11	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCen12	208 ± 25 nm	726 ± 20 nm	NB^b	742 ± 31 nm	NB^b
LigBCen2	212 ± 34 nm	ND^a	ND^a	ND^a	ND^a
LigBCen2NR	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCen3	NB^b	ND^a	ND^a	ND^a	ND^a
LigBCtv	NB^b	ND^a	ND^a	ND^a	ND^a
rFnBPA_194–511	42 ± 1.5 nm	133 ± 22 nm	ND^a	ND^a	ND^a

^a ND indicates not determined.

^b NB indicates no binding.