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Syt2 is expressed in mature MCs. Peritoneal MCs purified from P16 Syt2+/+ and Syt2−/− mice. A, immunoblot of cell lysates with anti-Syt2 antibody (αSyt2). GAPDH immunoreactivity was used as a loading control. B, immunohistochemistry of cytospins using the same antibody (bar, 4 μm). We demonstrated the presence of Syt2 in mature wild type MCs and the absence of Syt2 in Syt2−/− MCs.
