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. 2009 May 27;284(29):19445–19451. doi: 10.1074/jbc.M109.002550

FIGURE 4.

FIGURE 4.

Syt2 deficiency affects MC-regulated exocytosis. BMMCs from Syt2+/+ and Syt2−/− mice were incubated with anti-DNP IgE and then exposed to DNP-HSA. Secreted products were measured in supernatants before and 1 h after stimulation. Total β-hexosaminidase enzymatic activity was also measured in cell lysates. A, secretion of β-hexosaminidase with different doses of DNP-HSA. Values are expressed as the difference in enzymatic activity in supernatants between stimulated and unstimulated cells in percent of total activity in cell lysates. The maximal response and greatest difference were found at 100 ng/ml DNP-HSA (n = 3). This concentration was used in all subsequent experiments. B, secretion of β-hexosaminidase at 100 ng/ml DNP-HSA (n = 8). C, histamine in supernatants before (open bars) and after (black bars) challenge (n = 5). Release of products stored in MC secretory granules and secreted via regulated exocytosis was significantly decreased in Syt2−/− BMMCs. (Error bars represent S.E.; *, p < 0.01.)