TABLE 1.

Quantitation, morphometry, and cellular contents of studied MCs

Values obtained from four animals of each genotype are expressed as mean ± S.E. V_v denotes granule volume fraction, S_v, granule surface density, and A, cell profile area.

	Syt2+/+	Syt2−/−
Dermal MCsa
106 cells/m2	183 ± 15	182 ± 12
Peritoneal MCsb
%	9.7 ± 1.7	9.3 ± 1.1
106 cells/liter	180 ± 13	174 ± 16
Peritoneal MCsc
Vv	0.25 ± 0.02	0.26 ± 0.02
Sv, 10−6 m−1	22.89 ± 1.71	21.82 ± 2.85
A, 10−12 m2	33.82 ± 2.97	32.76 ± 0.78
BMMCs in cultured
2 weeks, %	17.2 ± 3.1	19.1 ± 3.9
4 weeks, %	89.1 ± 4.4	86.2 ± 5.4
6 weeks, %	97.5 ± 1.2	97.1 ± 1.4
BMMCs (6 w)c
Vv	0.41 ± 0.04	0.39 ± 0.03
Sv, 10−6 m−1	20.77 ± 2.01	20.46 ± 1.14
A, 10−12 m2	43.11 ± 2.88	48.58 ± 2.78
BMMCs histaminee
10−3 g/liter	0.93 ± 0.11	0.97 ± 0.09

^a FITC-avidin⁺ cells with a Hoechst⁺ nuclear profile per area of dermis in random sections of ear (10 samples/animal).

^b Calculated from Neubauer chamber counts and Wright-Giemsa staining of cytospins of peritoneal lavages (3 samples/animal).

^c Stereological analysis of randomly acquired EM images from peritoneal MCs and BMMCs after 6 weeks in culture (10 cell profiles/sample, 4 samples/animal).

^d Percent of Kit⁺/FcϵRIα⁺ double positive cells in bone marrow cultures in medium enriched with SCF and IL-3 assessed by flow cytometry (1 sample/animal/time point).

^e Total histamine concentration in lysates of 10⁶ BMMCs measured by ELISA (1 sample/animal).