FIGURE 3.

RPB9 deletion causes a uniform 3-fold increase of different types of misincorporation. WT or pol IIΔ9 TEC8 obtained from TEC7 on template 45C was incubated with 10 μm ATP (TEC9) for 30 s, after which 0.1 mm CTP (A), 0.1 mm UTP (B), 10 μm dCTP (C), 1 mm ATP (D), or 1 mm GTP (E) was added. The reaction with CTP was stopped with 1 m HCl. The experimental data set obtained for the WT (black) and pol IIΔ9 (red) are represented as in Fig. 1C. Error bars indicate S.E. Panel E shows GTP misincorporation.