FIGURE 2.

CT-1 represses the expression of pro-differentiation transcriptional regulators (MyoG and MEF2A/D). C2C12 cells were induced to differentiate in DM with CT-1 (10 ng/ml) or solvent. The cells were maintained in the indicated conditions for specific time periods. Total protein samples were extracted from the cells and equal amounts of total protein (20 μg) were subjected to Western blotting analysis. The levels of indicated proteins were assessed by a standard immuno-blotting technique with a specific primary antibody. Actin indicates equal amounts of protein loading into each lane.