FIGURE 4.
Activation of purified proHP6Xa and proHP6Xa,I by bovine Factor Xa. After incubation of the purified recombinant proHP6 proteins (50 ng/each) with Factor Xa (200 ng), the mixtures were separated by 10% SDS-PAGE followed by immunoblot analysis using HP6 antiserum. The sizes and positions of molecular weight standards are indicated on the left. These were compared with natural HP6 in plasma activated by exposure to M. luteus (far right lane). Bands representing proHP6 zymogen, a cleavage product corresponding to its catalytic domain, and a putative HP6-serpin complex are marked by arrows.