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. 2009 May 18;284(29):19727–19743. doi: 10.1074/jbc.M109.019844

FIGURE 2.

FIGURE 2.

HIV-1 RNA and Gag are transported to late endosomes. A, two patterns of HIV-1 RNAs localization occur in HT*A cells. pTRIP-MS2×24 and TiVamp-CFP were co-transfected and HIV-1 RNAs were detected by FISH. Red, HIV-1 RNA (RNA-Cy3); green, TiVamp (TiVamp-CFP). Images correspond to a projection of three-dimensional stacks (60 × 60 μm). Insets show magnification of the boxed areas (7 × 7 μm). Top, HIV-1 RNAs accumulate in late endosomes; bottom, HIV-1 RNAs are diffusely distributed in the cytoplasm. B, two patterns of HIV-1 Gag localization occur in HT*A cells. HIV-1 Gag was detected by immunofluorescence with an antibody against CA(p24). Late endosomes were revealed with transiently expressed TiVamp-CFP. Red, HIV-1 Gag (Gag-Cy3); green, TiVamp (TiVamp-CFP). Images correspond to a projection of three-dimensional stacks (47 × 45 μm). Insets show the magnification of the boxed areas (5 × 5 μm). Top, HIV-1 Gag accumulates in late endosomes; bottom, HIV-1 Gag is diffusely distributed in the cytoplasm. The arrow points to a site of cellular contact.