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Sensitivity of E. coli strains to hydroxylamine. Cells were plated on minimal medium plates using a multi-prong replicator, and 150 μg of hydroxylamine (in water) was spotted onto the center of each plate. Plates were incubated overnight. See Experimental procedures for details. Strains used were NR10836 (wt), NR12383 (moeA), NR15873 (ycbX), and NR15871 (yiiM). When replicated to LB-Rif plates, no induction of Rifr mutants was observed for any of the strains (not shown).
