Table 1.

Primer and probe sequences used in SNP detection

SNP	ref SNP ID#	Major allele	Minor allele	Location	Sequence**(5′ to 3′)
					Forward primer
					Reverse primer
					Probe (wild-type)
					Probe (variant)
TLR4 (A896G)	rs4986790	A	G	Exon 3	TGACCATTGAAGAATTCCGATTAGCA
					ACACTCACCAGGGAAAATGAAGAA
					FAM-TACCTCGATGATATTATT-MGB
					VIC-CCTCGATGGTATTATT-MGB
TNF-α (G-308A)	1800629	G	A	Promoter	CCAAAAGAAATGGAGGCAATAGGTT
					GGACCCTGGAGGCTGAAC
					FAM-CCCGTCCTCATGCC-MGB
					VIC-CCCGTCCCCATGCC-MGB
IL-6 (G-174C)	rs1800795	G	C	Promoter	GACGACCTAAGCTGCACTTTTC
					GGGCTGATTGGAAACCTTATTAAGATTG
					FAM-CTTTAGCATCGCAAGAC-MGB
					VIC-CCTTTAGCATGGCAAGAC-MGB
IL-1β(T-31C)	1143627	T	C	Promoter	CAGCCTCCTACTTCTGCTTTTGA
					AGGTTTGGTATCTGCCAGTTTCTC
					VIC-TCGCTGTTTTTATAGCTT-MGB
					FAM-CGCTGTTTTTATGGCTT-MGB
CD-14 (C-159T)	2569190	C	T	Promoter	CTAGATGCCCTGCAGAATCCTT
					CCCTTCCTTTCCTGGAAATATTGCA
					VIC-CTGTTACGGCCCCCCT-MGB
					FAM-CTGTTACGGTCCCCCT-MGB

TLR4, Toll-like receptor 4; TNF-α, tumor necrosis factor alpha; IL, interleukin; CD-14, cluster of differentiation marker 14.

MGB, minor groove binder

^*Polymorphic nucleotide is underlined.