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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Circ Res. 2009 Apr 2;104(9):1058–1065. doi: 10.1161/CIRCRESAHA.109.194506

Figure 4.

Figure 4

Elevated TNF-α expression in ischemic retina in APN-KO mice. A, Gene expression levels of TNF-α in retina at P13. Retinas were harvested from eyes of WT (n=7) or APN-KO (n=8) mice at P13. TNF-α mRNA levels were determined by quantitative real-time PCR methods and expressed relative to β-actin mRNA levels. Data are presented as means±SEM. B, TNF-α protein expression in ischemic retina in WT and APN-KO mice at P14. TNF-α protein levels were determined by ELISA. Data are presented as means±SEM (n=8). C and D, Adiponectin supplementation reduces retinal TNF-α mRNA (C) and protein (D) levels at P14 in WT and APN-KO mice. Ad-APN (4×107 pfu total) or Ad-βgal (control) was delivered intravenously at P10. TNF-α mRNA levels were measured by quantitative real-time PCR methods and expressed relative to β-actin mRNA levels. TNF-αprotein levels were determined by ELISA. Data are presented as means±SEM (n=6).