Fig. 4.
Effect of PARP inhibition by TIQ-A on airway hyperreactivity in OVA-sensitized and challenged mice. C57BL/6 mice were sensitized to OVA and subjected to a challenge with aerosolized OVA (∎). A group of mice received an i.p. injection of TIQ-A (▴) or vehicle alone (●)after OVA exposure. Lung resistance to increasing doses of MeCh was assessed using the forced oscillation technique. Lung resistance is plotted as a function of increasing doses of inhaled MeCh, using single compartment model and expressed as mean ± SEM. n = 5 per group. *Difference from challenged mice without TIQ-A treatment, P < 0.01. PARP, poly(ADP-ribose) polymerase; TIQ-A, thieno[2,3-c]isoquinolin-5-one; BAL, bronchio-alveolar lavage; MeCh, methacholine; OVA, ovalbumin; i.p. intraperitoneally.