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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Am J Physiol Cell Physiol. 2007 Jul 18;293(3):C1171–C1180. doi: 10.1152/ajpcell.00535.2006

Fig. 4.

Fig. 4

Effect of ouabain on ERK phosphorylation. OK cells were treated for 15 min with ouabain. The cell lysates were subjected to 10% SDS-PAGE, transferred, and probed for p-ERK1/2. The nitrocellulose membranes were stripped and reprobed for total ERK. Phosphorylated and total protein band densities were analyzed by densitometry, and the results (means ± SE from 3 independent experiments) are presented as the ratio of phosphorylated to total protein band density. *P < 0.05 by Student’s t-test.