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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Nat Med. 2008 Feb 24;14(3):306–314. doi: 10.1038/nm1716

Figure 4.

Figure 4

Notch regulation of bone marrow mesenchymal progenitors. (a) Bone marrow CFU-F assays for wild type (WT) versus PNN mutant littermates. (b) AP staining after 8 days in osteogenic medium following CFU-F assays. (c) AP quantitative assay for high-density BMSCs cultures in osteogenic medium. (d) Real-time PCR of osteoblast markers in BMSCs from 15-week-old mice cultured for 10 days in regular medium. (e) Phase-contrast pictures of high-density BMSCs cultures in adipogenic medium. Note lipid droplets only in wild type cells. (f) CFU-F assays for wild type BMSCs with or without 1 μM DAPT. Arrow denotes a typical “type I” CFU-F. (g) AP staining immediately following CFU-F assays of wild type BMSCs in f. (h) Representative “type II” CFU-Fs from g. *: p<0.05, n=3.